Reversible terminators are available with the 3'-ONH₂ protected as the acetoxime, which are more suitable for long term storage than our ready-to-use reversible terminators. For enzymatic incorporation, the oxime must be deprotected to the free 3'-ONH₂ before use, which can be achieved in situ with buffered aqueous methoxylamine.

	Thymine TONH2-101 5 µmoles $900.00
	Cytosine CONH2-102 5 µmoles $1050.00
	Adenine AONH2-103 5 µmoles $1050.00
	Guanine GONH2-104 5 µmoles $1050.00

Please inquire for bulk pricing and availability.

Polymerase variants have been developed that incorporate these terminators with improved efficiency over standard Taq DNA polymerase:

400 units = 10 nmoles (in ~80 µL). 1000 units = 25 nmoles (in ~200 µL). Estimated by UV @ 280 nm with e = 70360
Specific activity (nmol dNTP added/nmol pol) = 12 sec-1