Nucleic acid amplification reactions commonly suffer from the formation of off-target amplification products. This problem increases with the number of primers in the reaction, and mainly occurs because of primer-primer interactions.
SAMRSTM can solve this problem.
Selectively removing hydrogen bonding units from the primer, by replacing standard bases with SAMRS, results in self-avoiding DNA primers that can be added to enable and improve multiplexed PCR.
SAMRS bases can be incorporated into most multiplex PCR reactions where minimizing off-target binding is desired, to improve the performance and sensitivity for a wide variety of multiplex PCR purposes, including diagnostic panels.
To inquire about using SAMRSTM in your assay, please contact us.
As shown below, SAMRS bases, indicated with a *, can base pair with standard bases (in the target or amplicon) but not with other SAMRS bases.
Hoshika et al., 2010