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Oxime blocked untagged reversible terminators

Reversible terminators are available with the 3'-ONH2 protected as the acetoxime, which are more suitable for long term storage than our ready-to-use reversible terminators. For enzymatic incorporation, the oxime must be deprotected to the free 3'-ONH2 before use, which can be achieved in situ with buffered aqueous methoxylamine.

 

 

Thymine
TONH2-101

5 µmoles $900.00

 

 

Cytosine
CONH2-102

5 µmoles $1050.00

 

 

Adenine
AONH2-103

5 µmoles $1050.00

 

Guanine
GONH2-104

5 µmoles $1050.00

 

 

Please inquire for bulk pricing and availability.

 


 

Polymerase variants have been developed that incorporate these terminators with improved efficiency over standard Taq DNA polymerase:

 

POL475-400 400 units             $110.00
POL475-1000 1000 units            $280.00

 

400 units = 10 nmoles (in ~80 µL). 1000 units = 25 nmoles (in ~200 µL). Estimated by UV @ 280 nm with e = 70360
Specific activity (nmol dNTP added/nmol pol) = 12 sec-1