Reversible terminators are available with the 3'-ONH2 protected as the acetoxime, which are more suitable for long term storage than our ready-to-use reversible terminators. For enzymatic incorporation, the oxime must be deprotected to the free 3'-ONH2 before use, which can be achieved in situ with buffered aqueous methoxylamine.
 | Thymine TONH2-101 |
 | Cytosine CONH2-102 |
 | Adenine AONH2-103 |
 | Guanine GONH2-104 |
Please inquire for bulk pricing and availability.
Polymerase variants have been developed that incorporate these terminators with improved efficiency over standard Taq DNA polymerase:
POL475-400 | 400 units | $110.00 |
POL475-1000 | 1000 units | $280.00 |
400 units = 10 nmoles (in ~80 µL). 1000 units = 25 nmoles (in ~200 µL). Estimated by UV @ 280 nm with e = 70360
Specific activity (nmol dNTP added/nmol pol) = 12 sec-1